rabbit anti pea15  (Cell Signaling Technology Inc)

Journal: iScience

Article Title: PEA15 promotes osteosarcoma progression and cisplatin resistance by modulating autophagy through the FABP3-TNF signaling axis

doi: 10.1016/j.isci.2025.113695

Figure Lengend Snippet: Clinical characteristics and prognostic factor PEA15 in the OS autophagy-related model (A) Kaplan-Meier survival analysis of the OS prognostic factor PEA15. (B) Risk survival status plot. (C and D) Univariate and multivariate COX regression analyses of the OS autophagy-related model and various clinical features. (E) mRNA expression levels of PEA15 in the normal osteoblast cell line hFOB1.19 and four osteosarcoma cell lines. (F and G) Validation of knockdown efficiency in U2OS cells and overexpression efficiency in MG63 cells ( n = 3). (H and I) CCK-8 assays of cell proliferation following PEA15 knockdown and overexpression ( n = 3). (J–M) Validation of autophagy activity in human tumor tissues and corresponding statistical analysis ( n = 3). (N and O) Protein expression levels of PEA15 are validated by knockdown and overexpression experiments. The data are presented as mean ± SD. ∗∗ p < 0.01, ∗∗∗ p < 0.001. n.s.: not significant.

Article Snippet: The membrane was blocked with 5% non-fat milk in TBST for 2 h at room temperature, followed by incubation overnight at 4°C with primary antibodies targeting PEA15 (CST, #13091,1:1000), LC3B (CST, #3868,1:1000), ATG5 (CST,#9980,1:1000),Cleaved-Caspase3 (CST,#9664,1:1000),Cleaved-PARP (CST, #9541,1:1000), FABP3 (CST,#14780,1:1000), TNF-α (CST,#3707,1:1000), and GAPDH (CST, #5174,1:1000).

Techniques: Expressing, Biomarker Discovery, Knockdown, Over Expression, CCK-8 Assay, Activity Assay

Journal: iScience

Article Title: PEA15 promotes osteosarcoma progression and cisplatin resistance by modulating autophagy through the FABP3-TNF signaling axis

doi: 10.1016/j.isci.2025.113695

Figure Lengend Snippet: PEA15 influences tumor cell growth (A and B) Wound healing assays to evaluate the effects of PEA15 knockdown and overexpression. (C and D) Colony formation assays to assess the impact of PEA15 knockdown and overexpression. (E–G) Migration and invasion assays in U2OS cells with PEA15 knockdown, including statistical analysis and expression levels of migration- and invasion-related proteins ( n = 3). (H–J) Migration and invasion assays in MG63 cells with PEA15 overexpression, along with the corresponding protein expression profiles ( n = 3). The data are presented as mean ± SD. ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: The membrane was blocked with 5% non-fat milk in TBST for 2 h at room temperature, followed by incubation overnight at 4°C with primary antibodies targeting PEA15 (CST, #13091,1:1000), LC3B (CST, #3868,1:1000), ATG5 (CST,#9980,1:1000),Cleaved-Caspase3 (CST,#9664,1:1000),Cleaved-PARP (CST, #9541,1:1000), FABP3 (CST,#14780,1:1000), TNF-α (CST,#3707,1:1000), and GAPDH (CST, #5174,1:1000).

Techniques: Knockdown, Over Expression, Migration, Expressing

Journal: iScience

Article Title: PEA15 promotes osteosarcoma progression and cisplatin resistance by modulating autophagy through the FABP3-TNF signaling axis

doi: 10.1016/j.isci.2025.113695

Figure Lengend Snippet: PEA15 modulates autophagy and apoptosis (A and B) Assessment of autophagic activity (autophagosome formation) following PEA15 knockdown and overexpression ( n = 3). (C and D) Apoptosis assays were carried out to evaluate the effects of PEA15 knockdown and overexpression ( n = 3). (E and F) Impact of PEA15 knockdown and overexpression on the expression of autophagy-related proteins. (G and H) Changes in apoptosis-related protein levels after PEA15 knockdown and overexpression. The data are presented as mean ± SD. ∗∗ p < 0.01.

Article Snippet: The membrane was blocked with 5% non-fat milk in TBST for 2 h at room temperature, followed by incubation overnight at 4°C with primary antibodies targeting PEA15 (CST, #13091,1:1000), LC3B (CST, #3868,1:1000), ATG5 (CST,#9980,1:1000),Cleaved-Caspase3 (CST,#9664,1:1000),Cleaved-PARP (CST, #9541,1:1000), FABP3 (CST,#14780,1:1000), TNF-α (CST,#3707,1:1000), and GAPDH (CST, #5174,1:1000).

Techniques: Activity Assay, Knockdown, Over Expression, Expressing

Journal: iScience

Article Title: PEA15 promotes osteosarcoma progression and cisplatin resistance by modulating autophagy through the FABP3-TNF signaling axis

doi: 10.1016/j.isci.2025.113695

Figure Lengend Snippet: Mechanistic insights into PEA15 downstream signaling (A) Heatmap of transcriptomic analysis in U2OS cells following PEA15 knockdown. (B) CCK-8 assay was adopted to assess cell proliferation after FABP3 knockdown in U2OS cells ( n = 3). (C) Colony formation assay in U2OS cells with FABP3 knockdown ( n = 3). (D and E) Migration and invasion assays in U2OS cells with FABP3 knockdown, along with the expression levels of related proteins ( n = 3). (F and G) Fluorescence imaging of autophagic activity and expression of autophagy-related proteins following FABP3 knockdown. (K–L) Flow cytometry analysis of apoptosis and expression of apoptosis-related markers after FABP3 knockdown ( n = 3). (M) KEGG pathway analysis of transcriptomic data from U2OS cells with PEA15 knockdown. (N) In U2OS cells with PEA15 knockdown, FABP3 was overexpressed, followed by the detection of ATG5 and LC3B protein expression levels.

Article Snippet: The membrane was blocked with 5% non-fat milk in TBST for 2 h at room temperature, followed by incubation overnight at 4°C with primary antibodies targeting PEA15 (CST, #13091,1:1000), LC3B (CST, #3868,1:1000), ATG5 (CST,#9980,1:1000),Cleaved-Caspase3 (CST,#9664,1:1000),Cleaved-PARP (CST, #9541,1:1000), FABP3 (CST,#14780,1:1000), TNF-α (CST,#3707,1:1000), and GAPDH (CST, #5174,1:1000).

Techniques: Knockdown, CCK-8 Assay, Colony Assay, Migration, Expressing, Fluorescence, Imaging, Activity Assay, Flow Cytometry

Journal: iScience

Article Title: PEA15 promotes osteosarcoma progression and cisplatin resistance by modulating autophagy through the FABP3-TNF signaling axis

doi: 10.1016/j.isci.2025.113695

Figure Lengend Snippet: Knockdown of PEA15 Enhances the Anticancer Efficacy of DDP (A) Colony formation assays in control, DDP-treated, Si-PEA15, and DDP/Si-PEA15 combined treatment groups. (B) Migration and invasion assay images for the four treatment groups. (C) Fluorescence imaging of autophagic activity in the four treatment groups. (D and E) Expression levels of migration/invasion-related proteins and autophagy-related proteins in the four treatment groups. (F) Knockdown of PEA15 enhances the anticancer effect of DDP through the TNF signaling pathway. (G) The protein expression of ATG5 and LC3B was assessed in U2OS cells treated with the TNF-α antagonist (R-7050).

Article Snippet: The membrane was blocked with 5% non-fat milk in TBST for 2 h at room temperature, followed by incubation overnight at 4°C with primary antibodies targeting PEA15 (CST, #13091,1:1000), LC3B (CST, #3868,1:1000), ATG5 (CST,#9980,1:1000),Cleaved-Caspase3 (CST,#9664,1:1000),Cleaved-PARP (CST, #9541,1:1000), FABP3 (CST,#14780,1:1000), TNF-α (CST,#3707,1:1000), and GAPDH (CST, #5174,1:1000).

Techniques: Knockdown, Control, Migration, Invasion Assay, Fluorescence, Imaging, Activity Assay, Expressing

Journal: iScience

Article Title: PEA15 promotes osteosarcoma progression and cisplatin resistance by modulating autophagy through the FABP3-TNF signaling axis

doi: 10.1016/j.isci.2025.113695

Figure Lengend Snippet: In Vivo Tumor Formation Assay (A) Images of solid tumors from control, DDP-treated, Sh-PEA15, and DDP/Sh-PEA15 combined treatment groups. (B and C) Statistical analysis of tumor volume and tumor weight in the four treatment groups ( n = 3). (D) Hematoxylin and eosin (H&E) staining of tumors from the four treatment groups ( n = 3). (E) Expression of Ki67 in tumors from the four treatment groups in the animal experiment. (F) Expression of LC3B in tumors from the four treatment groups in the animal experiment. The data are presented as mean ± SD. ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: The membrane was blocked with 5% non-fat milk in TBST for 2 h at room temperature, followed by incubation overnight at 4°C with primary antibodies targeting PEA15 (CST, #13091,1:1000), LC3B (CST, #3868,1:1000), ATG5 (CST,#9980,1:1000),Cleaved-Caspase3 (CST,#9664,1:1000),Cleaved-PARP (CST, #9541,1:1000), FABP3 (CST,#14780,1:1000), TNF-α (CST,#3707,1:1000), and GAPDH (CST, #5174,1:1000).

Techniques: In Vivo, Tube Formation Assay, Control, Staining, Expressing

Journal: Cell Systems

Article Title: Context Specificity in Causal Signaling Networks Revealed by Phosphoprotein Profiling

doi: 10.1016/j.cels.2016.11.013

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-phospho-PEA15 (Ser116) , Invitrogen , Cat#44-836G; RRID: AB_2533775.

Techniques: Transduction, Recombinant, Protease Inhibitor, Bicinchoninic Acid Protein Assay, Software, Inhibition, Modification

Journal: Cell Systems

Article Title: Context Specificity in Causal Signaling Networks Revealed by Phosphoprotein Profiling

doi: 10.1016/j.cels.2016.11.013

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-PEA15 , Cell Signaling Technology , Cat#2780; RRID: AB_2268149.

Techniques: Transduction, Recombinant, Protease Inhibitor, Bicinchoninic Acid Protein Assay, Software, Inhibition, Modification